Journal: Journal of visualized experiments : JoVE

Article Title: Automated imaging and analysis for the quantification of fluorescently-labeled macropinosomes

doi: 10.3791/62828

Figure Lengend Snippet: Materials

Article Snippet: ​ Name of Material/ Equipment Company Catalog Number Comments/Description 0.25% Trypsin Corning 25053CI 0.1% EDTA in HBSS w/o Calcium, Magnesium and Sodium Bicarbonate 1.5 ml Microcentrifuge tube Fisherbrand 05-408-129 10-cm Tissue culture dish Greiner Bio-One 664160 CELLSTAR 15 mL Centrifuge tube Fisherbrand 07-200-886 2 L Beaker Fisherbrand 02-591-33 24-well Tissue culture plate Greiner Bio-One 662160 CELLSTAR 25 mL Divided reagent reservoir Genesee Scientific Corporation 28-123 25 mL Reagent reservoir Genesee Scientific Corporation 28-121 500 ml Beaker Fisherbrand 02-591-30 6-cm Tissue culture dish Greiner Bio-One 628160 CELLSTAR 8-Channel aspiration adapter Integra Biosciences 155503 8-Channel aspiration adapter for standard tips Integra Biosciences 159024 95% Ethanol Decon Laboratories Inc 4355226 Ammonia-free glass cleaner Sparkle FUN20500CT Black 96-well high-content screening microplate PerkinElmer 6055300 CellCarrier-96 Ultra Cotton-tipped applicator Fisherbrand 23-400-101 Coverslips Fisherbrand 12-545-80 12 mm diameter Cytation 5 Cell Imaging Multi-Mode Reader Biotek CYT5FW DAPI Millipore Sigma 5.08741 Dextran 70 kDa - FITC Life Technologies D1822 Lysine-fixable Dextran 70 kDa - TMR Life Technologies D1819 DMSO Millipore Sigma D1435 DPBS Corning 21031CV Without Calcium and Magnesium Forceps Fine Science Tools 11251-20 Dumont #5 Formaldehyde, 37% Ricca Chemical RSOF0010-250A ACS Reagent Grade Glycerol Fisher BioReagents BP229-1 Hardening fluorescence mounting media Agilent Tech S302380-2 DAKO Hoechst 33342 Millipore Sigma B2261 Hydrochloric acid (HCl) Fisher Chemical A144-212 Certified ACS Plus, 36.5 to 38.0% Kimwipes Kimberly-Clark 34155 Miscroscope slides Fisherbrand 12-544-1 Premium plain glass Multichannel pipette Gilson FA10013 8 channels, 0.5–10 uL Multichannel pipette Gilson FA10012 12 channels, 20–200 uL Multichannel pipette Gilson FA10011 8 channels, 20–200 uL Parafilm M Pechiney PM996 Plastic wrap Kirkland Signature 208733 Stretch-Tite Silicone isolators Grace Bio Labs Inc 664107 13mm Diameter × 0.8mm Depth ID, 25mm × 25mm Slide adapter Biotek 1220548 Wash bottle Fisherbrand FB0340922C Open in a separate window Materials

Techniques: High Content Screening, Imaging, Fluorescence, Transferring